Construct: shRNA TRCN0000422980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015256.2-2735s21c1
DNA Barcode:
GGTGTATAGAATCATGCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSL6 (23305)
Gene Description:
acyl-CoA synthetase long chain family member 6
Transcript:
RefSeq NM_015256.2 (NON-CURRENT)
Match location:
Position 2735 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000422980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGTATAGAATCATGCTAAA
Hairpin Sequence:
5'-CCGG-GGTGTATAGAATCATGCTAAA-CTCGAG-TTTAGCATGATTCTATACACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGTATAGAATCATGCTAAACTCGAGTTTAGCATGATTCTATACACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGTATAGAATCATGCTAAACTCGAGTTTAGCATGATTCTATACACC-3'

Other clones with same target sequence:

(none)