Construct: shRNA TRCN0000423073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130901.1-526s21c1
DNA Barcode:
GAGATGCCAATCTACACATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OTUD7A (161725)
Gene Description:
OTU deubiquitinase 7A
Transcript:
RefSeq NM_130901.1 (NON-CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGCCAATCTACACATTC
Hairpin Sequence:
5'-CCGG-GAGATGCCAATCTACACATTC-CTCGAG-GAATGTGTAGATTGGCATCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGCCAATCTACACATTCCTCGAGGAATGTGTAGATTGGCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGCCAATCTACACATTCCTCGAGGAATGTGTAGATTGGCATCTC-3'

Other clones with same target sequence:

(none)