Construct: shRNA TRCN0000423102

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014836.4-1813s21c1
DNA Barcode:
ACGTTCTCGGACGTGACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RHOBTB1 (9886)
Gene Description:
Rho related BTB domain containing 1
Transcript:
RefSeq NM_014836.4 (NON-CURRENT)
Match location:
Position 1813 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423102)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTTCTCGGACGTGACATTT
Hairpin Sequence:
5'-CCGG-ACGTTCTCGGACGTGACATTT-CTCGAG-AAATGTCACGTCCGAGAACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTTCTCGGACGTGACATTTCTCGAGAAATGTCACGTCCGAGAACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTTCTCGGACGTGACATTTCTCGAGAAATGTCACGTCCGAGAACGT-3'

Other clones with same target sequence:

(none)