Construct: shRNA TRCN0000423125

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014479.3-1805s21c1
DNA Barcode:
GGCTCTTTGTTTAGGCCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMDEC1 (27299)
Gene Description:
ADAM like decysin 1
Transcript:
RefSeq NM_014479.3 (CURRENT)
Match location:
Position 1805 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423125)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTCTTTGTTTAGGCCTAAT
Hairpin Sequence:
5'-CCGG-GGCTCTTTGTTTAGGCCTAAT-CTCGAG-ATTAGGCCTAAACAAAGAGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTCTTTGTTTAGGCCTAATCTCGAGATTAGGCCTAAACAAAGAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTCTTTGTTTAGGCCTAATCTCGAGATTAGGCCTAAACAAAGAGCC-3'

Other clones with same target sequence:

(none)