Construct: shRNA TRCN0000423209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145313.2-1559s21c1
DNA Barcode:
ACGTGCACTAACTGGGTTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASGEF1A (221002)
Gene Description:
RasGEF domain family member 1A
Transcript:
RefSeq NM_145313.2 (NON-CURRENT)
Match location:
Position 1559 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTGCACTAACTGGGTTTAA
Hairpin Sequence:
5'-CCGG-ACGTGCACTAACTGGGTTTAA-CTCGAG-TTAAACCCAGTTAGTGCACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTGCACTAACTGGGTTTAACTCGAGTTAAACCCAGTTAGTGCACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTGCACTAACTGGGTTTAACTCGAGTTAAACCCAGTTAGTGCACGT-3'

Other clones with same target sequence:

(none)