Construct: shRNA TRCN0000423229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017640.4-3321s21c1
DNA Barcode:
TGGAACACTTTACCAAGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARMIL1 (55604)
Gene Description:
capping protein regulator and myosin 1 linker 1
Transcript:
RefSeq NM_017640.4 (NON-CURRENT)
Match location:
Position 3321 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAACACTTTACCAAGTTAA
Hairpin Sequence:
5'-CCGG-TGGAACACTTTACCAAGTTAA-CTCGAG-TTAACTTGGTAAAGTGTTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAACACTTTACCAAGTTAACTCGAGTTAACTTGGTAAAGTGTTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAACACTTTACCAAGTTAACTCGAGTTAACTTGGTAAAGTGTTCCA-3'

Other clones with same target sequence:

(none)