Construct: shRNA TRCN0000423241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138817.2-951s21c1
DNA Barcode:
CCCTCATTAGCATGGATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC7A13 (157724)
Gene Description:
solute carrier family 7 member 13
Transcript:
RefSeq NM_138817.2 (NON-CURRENT)
Match location:
Position 951 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCATTAGCATGGATTATG
Hairpin Sequence:
5'-CCGG-CCCTCATTAGCATGGATTATG-CTCGAG-CATAATCCATGCTAATGAGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCATTAGCATGGATTATGCTCGAGCATAATCCATGCTAATGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCATTAGCATGGATTATGCTCGAGCATAATCCATGCTAATGAGGG-3'

Other clones with same target sequence:

(none)