Construct: shRNA TRCN0000423265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014498.3-544s21c1
DNA Barcode:
ACAGATCAAGATTAGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOLIM4 (27333)
Gene Description:
golgi integral membrane protein 4
Transcript:
RefSeq NM_014498.3 (NON-CURRENT)
Match location:
Position 544 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGATCAAGATTAGAGAAAT
Hairpin Sequence:
5'-CCGG-ACAGATCAAGATTAGAGAAAT-CTCGAG-ATTTCTCTAATCTTGATCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGATCAAGATTAGAGAAATCTCGAGATTTCTCTAATCTTGATCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGATCAAGATTAGAGAAATCTCGAGATTTCTCTAATCTTGATCTGT-3'

Other clones with same target sequence:

(none)