Construct: shRNA TRCN0000423299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145136.4-684s21c1
DNA Barcode:
TTCCGTGAAAGAGGCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myocd (214384)
Gene Description:
myocardin
Transcript:
RefSeq NM_145136.4 (CURRENT)
Match location:
Position 684 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCGTGAAAGAGGCTATAAA
Hairpin Sequence:
5'-CCGG-TTCCGTGAAAGAGGCTATAAA-CTCGAG-TTTATAGCCTCTTTCACGGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCGTGAAAGAGGCTATAAACTCGAGTTTATAGCCTCTTTCACGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCGTGAAAGAGGCTATAAACTCGAGTTTATAGCCTCTTTCACGGAA-3'

Other clones with same target sequence:

(none)