Construct: shRNA TRCN0000423345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014946.3-1664s21c1
DNA Barcode:
TGTAATGGGTGCAACTAATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPAST (6683)
Gene Description:
spastin
Transcript:
RefSeq NM_014946.3 (CURRENT)
Match location:
Position 1664 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAATGGGTGCAACTAATAG
Hairpin Sequence:
5'-CCGG-TGTAATGGGTGCAACTAATAG-CTCGAG-CTATTAGTTGCACCCATTACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAATGGGTGCAACTAATAGCTCGAGCTATTAGTTGCACCCATTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAATGGGTGCAACTAATAGCTCGAGCTATTAGTTGCACCCATTACA-3'

Other clones with same target sequence:

(none)