Construct: shRNA TRCN0000423384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015493.6-2562s21c1
DNA Barcode:
AGATTGCGTCCATGCTGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KANK2 (25959)
Gene Description:
KN motif and ankyrin repeat domains 2
Transcript:
RefSeq NM_015493.6 (CURRENT)
Match location:
Position 2562 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTGCGTCCATGCTGTATT
Hairpin Sequence:
5'-CCGG-AGATTGCGTCCATGCTGTATT-CTCGAG-AATACAGCATGGACGCAATCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTGCGTCCATGCTGTATTCTCGAGAATACAGCATGGACGCAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTGCGTCCATGCTGTATTCTCGAGAATACAGCATGGACGCAATCT-3'

Other clones with same target sequence:

(none)