Construct: shRNA TRCN0000423396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004473.3-1540s21c1
DNA Barcode:
AGTGCGATCTTTGCCGCTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXE1 (2304)
Gene Description:
forkhead box E1
Transcript:
RefSeq NM_004473.3 (NON-CURRENT)
Match location:
Position 1540 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCGATCTTTGCCGCTGCT
Hairpin Sequence:
5'-CCGG-AGTGCGATCTTTGCCGCTGCT-CTCGAG-AGCAGCGGCAAAGATCGCACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCGATCTTTGCCGCTGCTCTCGAGAGCAGCGGCAAAGATCGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCGATCTTTGCCGCTGCTCTCGAGAGCAGCGGCAAAGATCGCACT-3'

Other clones with same target sequence:

(none)