Construct: shRNA TRCN0000423471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015678.4-3952s21c1
DNA Barcode:
CCCAATAGTAGTACATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBEA (26960)
Gene Description:
neurobeachin
Transcript:
RefSeq NM_015678.4 (CURRENT)
Match location:
Position 3952 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATAGTAGTACATCATTT
Hairpin Sequence:
5'-CCGG-CCCAATAGTAGTACATCATTT-CTCGAG-AAATGATGTACTACTATTGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATAGTAGTACATCATTTCTCGAGAAATGATGTACTACTATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATAGTAGTACATCATTTCTCGAGAAATGATGTACTACTATTGGG-3'

Other clones with same target sequence:

(none)