Construct: shRNA TRCN0000423532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020870.3-3292s21c1
DNA Barcode:
TGTTTACAAGGCTTAACTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3RF1 (57630)
Gene Description:
SH3 domain containing ring finger 1
Transcript:
RefSeq NM_020870.3 (NON-CURRENT)
Match location:
Position 3292 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTTACAAGGCTTAACTAAT
Hairpin Sequence:
5'-CCGG-TGTTTACAAGGCTTAACTAAT-CTCGAG-ATTAGTTAAGCCTTGTAAACA-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTTACAAGGCTTAACTAATCTCGAGATTAGTTAAGCCTTGTAAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTTACAAGGCTTAACTAATCTCGAGATTAGTTAAGCCTTGTAAACA-3'

Other clones with same target sequence:

(none)