Construct: shRNA TRCN0000423532
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020870.3-3292s21c1
- DNA Barcode:
- TGTTTACAAGGCTTAACTAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SH3RF1 (57630)
- Gene Description:
- SH3 domain containing ring finger 1
- Transcript:
- RefSeq NM_020870.3 (NON-CURRENT)
- Match location:
- Position 3292 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000423532)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGTTTACAAGGCTTAACTAAT
- Hairpin Sequence:
-
5'-CCGG-TGTTTACAAGGCTTAACTAAT-CTCGAG-ATTAGTTAAGCCTTGTAAACA-TTTTTGGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGTTTACAAGGCTTAACTAATCTCGAGATTAGTTAAGCCTTGTAAACATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGTTTACAAGGCTTAACTAATCTCGAGATTAGTTAAGCCTTGTAAACA-3'
Other clones with same target sequence:
(none)
