Construct: shRNA TRCN0000423551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032814.3-709s21c1
DNA Barcode:
TGATCTGCTGGCTCCAGAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNFT2 (84900)
Gene Description:
ring finger protein, transmembrane 2
Transcript:
RefSeq NM_032814.3 (NON-CURRENT)
Match location:
Position 709 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCTGCTGGCTCCAGAAAG
Hairpin Sequence:
5'-CCGG-TGATCTGCTGGCTCCAGAAAG-CTCGAG-CTTTCTGGAGCCAGCAGATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCTGCTGGCTCCAGAAAGCTCGAGCTTTCTGGAGCCAGCAGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCTGCTGGCTCCAGAAAGCTCGAGCTTTCTGGAGCCAGCAGATCA-3'

Other clones with same target sequence:

(none)