Construct: shRNA TRCN0000423554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033049.2-1595s21c1
DNA Barcode:
ACCAATGTACAAGCTATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MUC13 (56667)
Gene Description:
mucin 13, cell surface associated
Transcript:
RefSeq NM_033049.2 (NON-CURRENT)
Match location:
Position 1595 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAATGTACAAGCTATTATT
Hairpin Sequence:
5'-CCGG-ACCAATGTACAAGCTATTATT-CTCGAG-AATAATAGCTTGTACATTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAATGTACAAGCTATTATTCTCGAGAATAATAGCTTGTACATTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAATGTACAAGCTATTATTCTCGAGAATAATAGCTTGTACATTGGT-3'

Other clones with same target sequence:

(none)