Construct: shRNA TRCN0000423617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031481.1-1706s21c1
DNA Barcode:
ATGGTACTTTCGTTGTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A18 (83733)
Gene Description:
solute carrier family 25 member 18
Transcript:
RefSeq NM_031481.1 (NON-CURRENT)
Match location:
Position 1706 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGTACTTTCGTTGTATTAA
Hairpin Sequence:
5'-CCGG-ATGGTACTTTCGTTGTATTAA-CTCGAG-TTAATACAACGAAAGTACCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGTACTTTCGTTGTATTAACTCGAGTTAATACAACGAAAGTACCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGTACTTTCGTTGTATTAACTCGAGTTAATACAACGAAAGTACCAT-3'

Other clones with same target sequence:

(none)