Construct: shRNA TRCN0000423622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172004.2-475s21c1
DNA Barcode:
ATTACTGCTATGGTGAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLECL1 (160365)
Gene Description:
C-type lectin like 1
Transcript:
RefSeq NM_172004.2 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTACTGCTATGGTGAGATTT
Hairpin Sequence:
5'-CCGG-ATTACTGCTATGGTGAGATTT-CTCGAG-AAATCTCACCATAGCAGTAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTACTGCTATGGTGAGATTTCTCGAGAAATCTCACCATAGCAGTAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTACTGCTATGGTGAGATTTCTCGAGAAATCTCACCATAGCAGTAAT-3'

Other clones with same target sequence:

(none)