Construct: shRNA TRCN0000423681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021927.2-2335s21c1
DNA Barcode:
TCGTTGAAAGGGAGTAGTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUF1 (60558)
Gene Description:
GUF1 homolog, GTPase
Transcript:
RefSeq NM_021927.2 (NON-CURRENT)
Match location:
Position 2335 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGTTGAAAGGGAGTAGTTAG
Hairpin Sequence:
5'-CCGG-TCGTTGAAAGGGAGTAGTTAG-CTCGAG-CTAACTACTCCCTTTCAACGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGTTGAAAGGGAGTAGTTAGCTCGAGCTAACTACTCCCTTTCAACGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGTTGAAAGGGAGTAGTTAGCTCGAGCTAACTACTCCCTTTCAACGA-3'

Other clones with same target sequence:

(none)