Construct: shRNA TRCN0000423685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014675.3-5347s21c1
DNA Barcode:
GAACATGACCGCCAAGTACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CROCC (9696)
Gene Description:
ciliary rootlet coiled-coil, rootletin
Transcript:
RefSeq NM_014675.3 (NON-CURRENT)
Match location:
Position 5347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACATGACCGCCAAGTACTC
Hairpin Sequence:
5'-CCGG-GAACATGACCGCCAAGTACTC-CTCGAG-GAGTACTTGGCGGTCATGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACATGACCGCCAAGTACTCCTCGAGGAGTACTTGGCGGTCATGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACATGACCGCCAAGTACTCCTCGAGGAGTACTTGGCGGTCATGTTC-3'

Other clones with same target sequence:

(none)