Construct: shRNA TRCN0000423767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000216.2-752s21c1
DNA Barcode:
AGGTTAAGTGGTCCTCGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANOS1 (3730)
Gene Description:
anosmin 1
Transcript:
RefSeq NM_000216.2 (NON-CURRENT)
Match location:
Position 752 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTTAAGTGGTCCTCGAAAT
Hairpin Sequence:
5'-CCGG-AGGTTAAGTGGTCCTCGAAAT-CTCGAG-ATTTCGAGGACCACTTAACCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTTAAGTGGTCCTCGAAATCTCGAGATTTCGAGGACCACTTAACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTTAAGTGGTCCTCGAAATCTCGAGATTTCGAGGACCACTTAACCT-3'

Other clones with same target sequence:

(none)