Construct: shRNA TRCN0000423844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018214.4-625s21c1
DNA Barcode:
ACTGACTAGGTTGCCAGAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC1 (55227)
Gene Description:
leucine rich repeat containing 1
Transcript:
RefSeq NM_018214.4 (NON-CURRENT)
Match location:
Position 625 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGACTAGGTTGCCAGAAAG
Hairpin Sequence:
5'-CCGG-ACTGACTAGGTTGCCAGAAAG-CTCGAG-CTTTCTGGCAACCTAGTCAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGACTAGGTTGCCAGAAAGCTCGAGCTTTCTGGCAACCTAGTCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGACTAGGTTGCCAGAAAGCTCGAGCTTTCTGGCAACCTAGTCAGT-3'

Other clones with same target sequence:

(none)