Construct: shRNA TRCN0000423862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145236.2-1550s21c1
DNA Barcode:
GGACCTCCCTGTGTGGATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B3GNT7 (93010)
Gene Description:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Transcript:
RefSeq NM_145236.2 (NON-CURRENT)
Match location:
Position 1550 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACCTCCCTGTGTGGATAAT
Hairpin Sequence:
5'-CCGG-GGACCTCCCTGTGTGGATAAT-CTCGAG-ATTATCCACACAGGGAGGTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACCTCCCTGTGTGGATAATCTCGAGATTATCCACACAGGGAGGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACCTCCCTGTGTGGATAATCTCGAGATTATCCACACAGGGAGGTCC-3'

Other clones with same target sequence:

(none)