Construct: shRNA TRCN0000423889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005516.4-1297s21c1
DNA Barcode:
CACCTCTGTGTCTACCATGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HLA-E (3133)
Gene Description:
major histocompatibility complex, class I, E
Transcript:
RefSeq NM_005516.4 (NON-CURRENT)
Match location:
Position 1297 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTCTGTGTCTACCATGAC
Hairpin Sequence:
5'-CCGG-CACCTCTGTGTCTACCATGAC-CTCGAG-GTCATGGTAGACACAGAGGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTCTGTGTCTACCATGACCTCGAGGTCATGGTAGACACAGAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTCTGTGTCTACCATGACCTCGAGGTCATGGTAGACACAGAGGTG-3'

Other clones with same target sequence:

(none)