Construct: shRNA TRCN0000423962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000855.1-1998s21c1
DNA Barcode:
GTGTACTCCCATGCAAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUCY1A2 (2977)
Gene Description:
guanylate cyclase 1 soluble subunit alpha 2
Transcript:
RefSeq NM_000855.1 (NON-CURRENT)
Match location:
Position 1998 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTACTCCCATGCAAGTAAT
Hairpin Sequence:
5'-CCGG-GTGTACTCCCATGCAAGTAAT-CTCGAG-ATTACTTGCATGGGAGTACAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTACTCCCATGCAAGTAATCTCGAGATTACTTGCATGGGAGTACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTACTCCCATGCAAGTAATCTCGAGATTACTTGCATGGGAGTACAC-3'

Other clones with same target sequence:

(none)