Construct: shRNA TRCN0000423979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019045.3-2761s21c1
DNA Barcode:
AGTATAAGGGTTACGTCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR44 (54521)
Gene Description:
WD repeat domain 44
Transcript:
RefSeq NM_019045.3 (NON-CURRENT)
Match location:
Position 2761 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTATAAGGGTTACGTCAATA
Hairpin Sequence:
5'-CCGG-AGTATAAGGGTTACGTCAATA-CTCGAG-TATTGACGTAACCCTTATACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTATAAGGGTTACGTCAATACTCGAGTATTGACGTAACCCTTATACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTATAAGGGTTACGTCAATACTCGAGTATTGACGTAACCCTTATACT-3'

Other clones with same target sequence:

(none)