Construct: shRNA TRCN0000423995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207306.2-1713s21c1
DNA Barcode:
AGCGGCACTTTGATCCGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP73-AS1 (57212)
Gene Description:
TP73 antisense RNA 1
Transcript:
RefSeq NM_207306.2 (NON-CURRENT)
Match location:
Position 1713 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000423995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGGCACTTTGATCCGGTTT
Hairpin Sequence:
5'-CCGG-AGCGGCACTTTGATCCGGTTT-CTCGAG-AAACCGGATCAAAGTGCCGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGGCACTTTGATCCGGTTTCTCGAGAAACCGGATCAAAGTGCCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGGCACTTTGATCCGGTTTCTCGAGAAACCGGATCAAAGTGCCGCT-3'

Other clones with same target sequence:

(none)