Construct: shRNA TRCN0000424016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003986.2-1576s21c1
DNA Barcode:
TTGTGAGATATGGCACATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BBOX1 (8424)
Gene Description:
gamma-butyrobetaine hydroxylase 1
Transcript:
RefSeq NM_003986.2 (NON-CURRENT)
Match location:
Position 1576 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTGAGATATGGCACATTAT
Hairpin Sequence:
5'-CCGG-TTGTGAGATATGGCACATTAT-CTCGAG-ATAATGTGCCATATCTCACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTGAGATATGGCACATTATCTCGAGATAATGTGCCATATCTCACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTGAGATATGGCACATTATCTCGAGATAATGTGCCATATCTCACAA-3'

Other clones with same target sequence:

(none)