Construct: shRNA TRCN0000424038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018246.2-384s21c1
DNA Barcode:
GTATATACGCCGTGGTCTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC25 (55246)
Gene Description:
coiled-coil domain containing 25
Transcript:
RefSeq NM_018246.2 (NON-CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATATACGCCGTGGTCTAAC
Hairpin Sequence:
5'-CCGG-GTATATACGCCGTGGTCTAAC-CTCGAG-GTTAGACCACGGCGTATATAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATATACGCCGTGGTCTAACCTCGAGGTTAGACCACGGCGTATATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATATACGCCGTGGTCTAACCTCGAGGTTAGACCACGGCGTATATAC-3'

Other clones with same target sequence:

(none)