Construct: shRNA TRCN0000424054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005651.2-281s21c1
DNA Barcode:
ATCATAACTCATCAAGCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TDO2 (6999)
Gene Description:
tryptophan 2,3-dioxygenase
Transcript:
RefSeq NM_005651.2 (NON-CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATAACTCATCAAGCTTAT
Hairpin Sequence:
5'-CCGG-ATCATAACTCATCAAGCTTAT-CTCGAG-ATAAGCTTGATGAGTTATGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATAACTCATCAAGCTTATCTCGAGATAAGCTTGATGAGTTATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATAACTCATCAAGCTTATCTCGAGATAAGCTTGATGAGTTATGAT-3'

Other clones with same target sequence:

(none)