Construct: shRNA TRCN0000424112

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022098.2-1005s21c1
DNA Barcode:
GCTGGTGGTAATCGGTCAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPNPEP3 (63929)
Gene Description:
X-prolyl aminopeptidase 3
Transcript:
RefSeq NM_022098.2 (NON-CURRENT)
Match location:
Position 1005 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424112)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTGGTAATCGGTCAAAC
Hairpin Sequence:
5'-CCGG-GCTGGTGGTAATCGGTCAAAC-CTCGAG-GTTTGACCGATTACCACCAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTGGTAATCGGTCAAACCTCGAGGTTTGACCGATTACCACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTGGTAATCGGTCAAACCTCGAGGTTTGACCGATTACCACCAGC-3'

Other clones with same target sequence:

(none)