Construct: shRNA TRCN0000424115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152732.3-114s21c1
DNA Barcode:
ACGTCTCTTATGCTGGTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSPH9 (221421)
Gene Description:
radial spoke head component 9
Transcript:
RefSeq NM_152732.3 (NON-CURRENT)
Match location:
Position 114 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCTCTTATGCTGGTTAAG
Hairpin Sequence:
5'-CCGG-ACGTCTCTTATGCTGGTTAAG-CTCGAG-CTTAACCAGCATAAGAGACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCTCTTATGCTGGTTAAGCTCGAGCTTAACCAGCATAAGAGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCTCTTATGCTGGTTAAGCTCGAGCTTAACCAGCATAAGAGACGT-3'

Other clones with same target sequence:

(none)