Construct: shRNA TRCN0000424134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138807.2-607s21c1
DNA Barcode:
TCCTCAACTGGAATAACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAMM41 (132001)
Gene Description:
TAM41 mitochondrial translocator assembly and maintenance homolog
Transcript:
RefSeq NM_138807.2 (NON-CURRENT)
Match location:
Position 607 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTCAACTGGAATAACTTAT
Hairpin Sequence:
5'-CCGG-TCCTCAACTGGAATAACTTAT-CTCGAG-ATAAGTTATTCCAGTTGAGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTCAACTGGAATAACTTATCTCGAGATAAGTTATTCCAGTTGAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTCAACTGGAATAACTTATCTCGAGATAAGTTATTCCAGTTGAGGA-3'

Other clones with same target sequence:

(none)