Construct: shRNA TRCN0000424225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033029.3-766s21c1
DNA Barcode:
TGCTAACCTGTGTCCAAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMLN (89782)
Gene Description:
leishmanolysin like peptidase
Transcript:
RefSeq NM_033029.3 (CURRENT)
Match location:
Position 766 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTAACCTGTGTCCAAATAT
Hairpin Sequence:
5'-CCGG-TGCTAACCTGTGTCCAAATAT-CTCGAG-ATATTTGGACACAGGTTAGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTAACCTGTGTCCAAATATCTCGAGATATTTGGACACAGGTTAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTAACCTGTGTCCAAATATCTCGAGATATTTGGACACAGGTTAGCA-3'

Other clones with same target sequence:

(none)