Construct: shRNA TRCN0000424247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032552.2-496s21c1
DNA Barcode:
TTCGAGGTGACGACGTCATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAB2IP (153090)
Gene Description:
DAB2 interacting protein
Transcript:
RefSeq NM_032552.2 (NON-CURRENT)
Match location:
Position 496 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGAGGTGACGACGTCATCA
Hairpin Sequence:
5'-CCGG-TTCGAGGTGACGACGTCATCA-CTCGAG-TGATGACGTCGTCACCTCGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGAGGTGACGACGTCATCACTCGAGTGATGACGTCGTCACCTCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGAGGTGACGACGTCATCACTCGAGTGATGACGTCGTCACCTCGAA-3'

Other clones with same target sequence:

(none)