Construct: shRNA TRCN0000424285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004348.3-863s21c1
DNA Barcode:
CGGAATGCCTCTGCTGTTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RUNX2 (860)
Gene Description:
RUNX family transcription factor 2
Transcript:
RefSeq NM_004348.3 (NON-CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATGCCTCTGCTGTTATG
Hairpin Sequence:
5'-CCGG-CGGAATGCCTCTGCTGTTATG-CTCGAG-CATAACAGCAGAGGCATTCCG-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATGCCTCTGCTGTTATGCTCGAGCATAACAGCAGAGGCATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATGCCTCTGCTGTTATGCTCGAGCATAACAGCAGAGGCATTCCG-3'

Other clones with same target sequence:

(none)