Construct: shRNA TRCN0000424313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004498.1-1641s21c1
DNA Barcode:
AGCGTTATTTATAGTCCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ONECUT1 (3175)
Gene Description:
one cut homeobox 1
Transcript:
RefSeq NM_004498.1 (NON-CURRENT)
Match location:
Position 1641 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGTTATTTATAGTCCAAAG
Hairpin Sequence:
5'-CCGG-AGCGTTATTTATAGTCCAAAG-CTCGAG-CTTTGGACTATAAATAACGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGTTATTTATAGTCCAAAGCTCGAGCTTTGGACTATAAATAACGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGTTATTTATAGTCCAAAGCTCGAGCTTTGGACTATAAATAACGCT-3'

Other clones with same target sequence:

(none)