Construct: shRNA TRCN0000424351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177130.3-560s21c1
DNA Barcode:
CCTGAGCCATTCAGTACTGAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glt28d2 (320302)
Gene Description:
glycosyltransferase 28 domain containing 2
Transcript:
RefSeq NM_177130.3 (CURRENT)
Match location:
Position 560 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGCCATTCAGTACTGAG
Hairpin Sequence:
5'-CCGG-CCTGAGCCATTCAGTACTGAG-CTCGAG-CTCAGTACTGAATGGCTCAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGCCATTCAGTACTGAGCTCGAGCTCAGTACTGAATGGCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGCCATTCAGTACTGAGCTCGAGCTCAGTACTGAATGGCTCAGG-3'

Other clones with same target sequence:

(none)