Construct: shRNA TRCN0000424418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024953.2-3187s21c1
DNA Barcode:
GCAACAAGAGAACCCTACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAA25 (80018)
Gene Description:
N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Transcript:
RefSeq NM_024953.2 (NON-CURRENT)
Match location:
Position 3187 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAAGAGAACCCTACTTT
Hairpin Sequence:
5'-CCGG-GCAACAAGAGAACCCTACTTT-CTCGAG-AAAGTAGGGTTCTCTTGTTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAAGAGAACCCTACTTTCTCGAGAAAGTAGGGTTCTCTTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAAGAGAACCCTACTTTCTCGAGAAAGTAGGGTTCTCTTGTTGC-3'

Other clones with same target sequence:

(none)