Construct: shRNA TRCN0000424455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012262.3-867s21c1
DNA Barcode:
ATGTCATAAGGGATCCTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HS2ST1 (9653)
Gene Description:
heparan sulfate 2-O-sulfotransferase 1
Transcript:
RefSeq NM_012262.3 (NON-CURRENT)
Match location:
Position 867 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTCATAAGGGATCCTATTG
Hairpin Sequence:
5'-CCGG-ATGTCATAAGGGATCCTATTG-CTCGAG-CAATAGGATCCCTTATGACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTCATAAGGGATCCTATTGCTCGAGCAATAGGATCCCTTATGACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTCATAAGGGATCCTATTGCTCGAGCAATAGGATCCCTTATGACAT-3'

Other clones with same target sequence:

(none)