Construct: shRNA TRCN0000424477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175500.4-2490s21c1
DNA Barcode:
CAGAACACAATAAGTAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpc5 (103978)
Gene Description:
glypican 5
Transcript:
RefSeq NM_175500.4 (CURRENT)
Match location:
Position 2490 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACACAATAAGTAGTATT
Hairpin Sequence:
5'-CCGG-CAGAACACAATAAGTAGTATT-CTCGAG-AATACTACTTATTGTGTTCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACACAATAAGTAGTATTCTCGAGAATACTACTTATTGTGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACACAATAAGTAGTATTCTCGAGAATACTACTTATTGTGTTCTG-3'

Other clones with same target sequence:

(none)