Construct: shRNA TRCN0000424479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020876.1-887s21c1
DNA Barcode:
GAAAGGGAACGAGCCGTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP23 (57636)
Gene Description:
Rho GTPase activating protein 23
Transcript:
RefSeq NM_020876.1 (NON-CURRENT)
Match location:
Position 887 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGGGAACGAGCCGTATTC
Hairpin Sequence:
5'-CCGG-GAAAGGGAACGAGCCGTATTC-CTCGAG-GAATACGGCTCGTTCCCTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGGGAACGAGCCGTATTCCTCGAGGAATACGGCTCGTTCCCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGGGAACGAGCCGTATTCCTCGAGGAATACGGCTCGTTCCCTTTC-3'

Other clones with same target sequence:

(none)