Construct: shRNA TRCN0000424489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005021.3-2998s21c1
DNA Barcode:
TTATCTTACTCAGAATCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENPP3 (5169)
Gene Description:
ectonucleotide pyrophosphatase/phosphodiesterase 3
Transcript:
RefSeq NM_005021.3 (NON-CURRENT)
Match location:
Position 2998 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATCTTACTCAGAATCTTTG
Hairpin Sequence:
5'-CCGG-TTATCTTACTCAGAATCTTTG-CTCGAG-CAAAGATTCTGAGTAAGATAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATCTTACTCAGAATCTTTGCTCGAGCAAAGATTCTGAGTAAGATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATCTTACTCAGAATCTTTGCTCGAGCAAAGATTCTGAGTAAGATAA-3'

Other clones with same target sequence:

(none)