Construct: shRNA TRCN0000424516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178373.3-410s21c1
DNA Barcode:
CCCATCAGAACAGCGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cidec (14311)
Gene Description:
cell death-inducing DFFA-like effector c
Transcript:
RefSeq NM_178373.3 (CURRENT)
Match location:
Position 410 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCAGAACAGCGCAAGAA
Hairpin Sequence:
5'-CCGG-CCCATCAGAACAGCGCAAGAA-CTCGAG-TTCTTGCGCTGTTCTGATGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCAGAACAGCGCAAGAACTCGAGTTCTTGCGCTGTTCTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCAGAACAGCGCAAGAACTCGAGTTCTTGCGCTGTTCTGATGGG-3'

Other clones with same target sequence:

(none)