Construct: shRNA TRCN0000424648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032287.2-850s21c1
DNA Barcode:
AGAGTTGCGGAGAACCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTL6 (84247)
Gene Description:
retrotransposon Gag like 6
Transcript:
RefSeq NM_032287.2 (NON-CURRENT)
Match location:
Position 850 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTTGCGGAGAACCTACAA
Hairpin Sequence:
5'-CCGG-AGAGTTGCGGAGAACCTACAA-CTCGAG-TTGTAGGTTCTCCGCAACTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTTGCGGAGAACCTACAACTCGAGTTGTAGGTTCTCCGCAACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTTGCGGAGAACCTACAACTCGAGTTGTAGGTTCTCCGCAACTCT-3'

Other clones with same target sequence:

(none)