Construct: shRNA TRCN0000424749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005583.3-408s21c1
DNA Barcode:
AGAAGGCAGAGATGGTGTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYL1 (4066)
Gene Description:
LYL1 basic helix-loop-helix family member
Transcript:
RefSeq NM_005583.3 (NON-CURRENT)
Match location:
Position 408 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGGCAGAGATGGTGTGTG
Hairpin Sequence:
5'-CCGG-AGAAGGCAGAGATGGTGTGTG-CTCGAG-CACACACCATCTCTGCCTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGGCAGAGATGGTGTGTGCTCGAGCACACACCATCTCTGCCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGGCAGAGATGGTGTGTGCTCGAGCACACACCATCTCTGCCTTCT-3'

Other clones with same target sequence:

(none)