Construct: shRNA TRCN0000424769

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000701.7-3501s21c1
DNA Barcode:
GAACTCTACCCTGGTAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP1A1 (476)
Gene Description:
ATPase Na+/K+ transporting subunit alpha 1
Transcript:
RefSeq NM_000701.7 (NON-CURRENT)
Match location:
Position 3501 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424769)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTCTACCCTGGTAGGAAA
Hairpin Sequence:
5'-CCGG-GAACTCTACCCTGGTAGGAAA-CTCGAG-TTTCCTACCAGGGTAGAGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTCTACCCTGGTAGGAAACTCGAGTTTCCTACCAGGGTAGAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTCTACCCTGGTAGGAAACTCGAGTTTCCTACCAGGGTAGAGTTC-3'

Other clones with same target sequence:

(none)