Construct: shRNA TRCN0000424782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032680.3-1132s21c1
DNA Barcode:
AGAAGGAGGAACCTCATTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRACR2A (84766)
Gene Description:
calcium release activated channel regulator 2A
Transcript:
RefSeq NM_032680.3 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGGAGGAACCTCATTTAC
Hairpin Sequence:
5'-CCGG-AGAAGGAGGAACCTCATTTAC-CTCGAG-GTAAATGAGGTTCCTCCTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGGAGGAACCTCATTTACCTCGAGGTAAATGAGGTTCCTCCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGGAGGAACCTCATTTACCTCGAGGTAAATGAGGTTCCTCCTTCT-3'

Other clones with same target sequence:

(none)