Construct: shRNA TRCN0000424783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020750.2-1088s21c1
DNA Barcode:
GATGTTGCCATGCATTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPO5 (57510)
Gene Description:
exportin 5
Transcript:
RefSeq NM_020750.2 (NON-CURRENT)
Match location:
Position 1088 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTTGCCATGCATTATATA
Hairpin Sequence:
5'-CCGG-GATGTTGCCATGCATTATATA-CTCGAG-TATATAATGCATGGCAACATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTTGCCATGCATTATATACTCGAGTATATAATGCATGGCAACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTTGCCATGCATTATATACTCGAGTATATAATGCATGGCAACATC-3'

Other clones with same target sequence:

(none)