Construct: shRNA TRCN0000424825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031213.3-1408s21c1
DNA Barcode:
GCACAACGACATCGAGCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABHD17A (81926)
Gene Description:
abhydrolase domain containing 17A
Transcript:
RefSeq NM_031213.3 (NON-CURRENT)
Match location:
Position 1408 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000424825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAACGACATCGAGCTCTA
Hairpin Sequence:
5'-CCGG-GCACAACGACATCGAGCTCTA-CTCGAG-TAGAGCTCGATGTCGTTGTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAACGACATCGAGCTCTACTCGAGTAGAGCTCGATGTCGTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAACGACATCGAGCTCTACTCGAGTAGAGCTCGATGTCGTTGTGC-3'

Other clones with same target sequence:

(none)